Bioflocculant Synthesized by Klebsiella pneumoniae during 1,3-Propanediol and Bio-diesel Integration Process

摘要

The bio-diesel and 1,3-propanediol (1,3-PD) processes are combined in our practices. The properties of flocculant from 1,3-propanediol fermentation was investigated. The characterization of the bioflocculant by GPC indicated the average molecular weight was about 2.0×10~5 Da. The neutral mono saccharides were the main component of the flocculant. Ca~(2+), Mg~(2+), Cu~(2+), K~+ and Na~+ were employed as flocculant aid to investigate the flocculating activity with Kaolin suspension. Ca~(2+) and Mg~(2+) provided the significant positive effect whereas Cuz+ provided a negative effect. 3.4 mmol/l Ca~(2+) was chosen for the further research. Two peaks of flocculating activity appeared at about pH 4.5 and 10, respectively. A case study of dye removal showed that the by-product flocculant from of 1,3-PD fermentation was a promising material for discoloring of textile wastewater.