Development of the Micro-Silk Through the Breeding of Transgenic Silkworm

摘要

The purpose of this research is to develop the micro-silk through the breeding of the transgenic silkworm. First of all, to construct transformation vector system of silkworm, we did cloning the sericin promoter in silkworm and sorting the selection marker gene (in order that sorting the transformation silkworm) and then cloning again. Second, we analyzed the fibroin gene of silkworm and IGF-1 gene of human After cloning. Then, we made and analyzed transformants of silkworm which gene expression as IGF-1 of human and the fibroin of silkworm. We inserted the protein expression vector which IGF-1 of human and the fibroin of silkworm into silkworm eggs using the microinjection. Using PCR and Southern blot methods, we analyzed expression vector in DNA level. In addition, we analyzed it using the RT-PCR methods in order to confirm gene expression in RNA level. The protein expression was analyzed in protein level through the western blot and ELISA methods. Through transformation on two separate occasions on spring and summer, we determined growth and ecology between Uzbekistan silkworm and Korean transformation silkworm. Lastly, we compared fabric of Uzbekistan silk, Chinese silk and Japanese 6A which we developed. Also we analyzed sericin content, strength and elongation and thickness of transformation silk single yarn.