Development of Immunochromatographic Test Strip based on Quantum Dot Nanoparticles(QDs)

摘要

Water-soluble fluorescent CdSe/CdZnS quantum dots(QDs)(λ_(cm)=637nm)were used as a labelling reporter for strip detection.The CdSe/CdZnS QDs was prepared by using core shell technique where CdSe QDs(λ_(cm)=590nm)core was capped with CdZnS shell.CdZnS-QDs were conjugated with amine-modified DNA probe,that can bio-recognize with DNA product for use in lateral flow assays(LFAs)detection.The presence of functionalized water-dispersible QDs was confirmed by Fourier transform infra-red(FTIR)technique,where a characteristic carboxylic group was observed at 1633 cm~(-1)(-OH)and 3310 cm~(-1)(-COO).DNA probe was immobilized onto QDs by EDC/NHS pathways and confirmed by protein absorption spectra at 260 nm due to heterocyclic ring of nucleosides.QDs-DNA can be applied as labelling reporter to readout fluorescence signal on the LFAs detection.