Automated High-Content Phenotyping of the Nematode C. Elegans at Single Animal Resolution with a Microfluidic Platform

摘要

In this work, we present a novel microfluidic approach to culture the nematode C. elegans from the onset of embryogenesis to the adult stage at single animal resolution. Our device allows high-throughput experimentation, high-resolution imaging, thereby providing high-content phenotyping in a highly parallel fashion. As a validation study, we studied the dose-dependent influence of the anthelmintic drug, tetramisole on the nematodes’ development during their full life cycle. Our results revealed a clear impact of tetramisole on various worm phenotypes.