Discrimination between primary low and high grade tumor and secondary metastasis tumor from deep-UV to NIR

摘要

Among Central Nervous System (CNS) tumors, diffuse glioma are the most infiltrating and malignant tumors. Accordingto the World Health Organization (WHO), they are classified into different grades, referring to their pathological class andhistological properties. To treat glioma tumors, many methods have been proposed, still the standard one remains themaximal safe total resection. During the operation, many difficulties obstruct the surgeon to identify the infiltrated areas,which contains diffuse tumors cells, around the solid area of the tumor, overlapping the healthy areas, and presenting thesame visual appearances. If not totally removed, these infiltrating zones can increase the risk of recurrence and affects thesurvival rate of patient. To overcome this problem, we develop a multimodal two-photon endomicroscope, based on theendogenous fluorescence of brain tissues, to assist the surgeon during the surgery. The tool will provide him informationon the infiltrated areas and their histological nature. In this paper, we tried to discriminate between metastasis, low gradeand high grade glioma from healthy fresh tissues, presenting a multimodal study using deep ultraviolet, visible and nearinfrared excitation to acquire spectral measurements, Fluorescence Lifetime Imaging (FLIM) and Two-Photon EmissionFluorescence (TPEF) imaging. We compared also our TPEF and FLIM images to the histological images.