Alkaloids piperine in dichloromethane fraction of red galangal rizhome(Alpinia purpurata)

摘要

Red galangal rizhome(Alpinia purpurata)was extracted with dichloromethane using maceration methods.The crude extract was fractionated using vacuum column chromatography with a mixture of ethyl acetate:n-hexane(2:3)to give two fractions(F1 and F2).Fraction 2 was fed into the silica gel of column chromatography tailed by recrystallization to produce crystalline(17 mg).The purity was tested by using melting point apparatus and thin-layer chromatography method for any solvent.Their structures were established by spectroscopic methods(UV-Vis,FTIR,’H-NMR and ~(13)C-NMR).Result of analysis gave melting point of crystals around 130°C.The UV-Vis spectrometer obtained maximum wavelength 344 nm.FTIR analysis showed C=O carbonyl,C-O ether,C-N,and C=C aromatic.Analysis by ~1H-NMR spectrometer showed that bioactive compounds have a 19 protons.Analysis by ~(13)C-NMR spectrometer showed that bioactive compounds have a 17 carbon atoms.The results of identification showed that bioactive compounds in dichloromethane fraction of red galangal rhizome was a compound piperine alkaloids types with a chemical formula of C_(17)H_(19)O3N.