A Simple Approach for Obtaining High Resolution, High Sensitivity 1{sup left}H NMR Metabolite Spectra of Biofluids with Limited Mass Supply

摘要

A simple approach is reported that yields high resolution, high sensitivity 1{sup left}H NMR spectra of intact biofluids with limited mass supply. This is achieved by spinning a capillary sample tube containing a biofluid at the magic angle and at a spinning rate of about 80Hz. A 2D pulse sequence called 1{sup left}H PASS is used to produce a high-resolution 1{sup left}H NMR spectrum that is free from magnetic susceptibility induced line broadening. Using this new approach a high resolution 1{sup left}H NMR spectrum of biofluids with a volume less than 1.0 μl can be achieved at a magnetic field strength as low as 7.05T. Furthermore, the methodology facilitates easy sample handling and is suitable for high throughput investigations. In this paper first results are shown obtained at a magnetic field of 7.05T on urine samples collected from mice using a modified commercial NMR probe.