HOTSPOT WIZARD 3.0: AUTOMATED DESIGN OF SITE-SPECIFIC MUTATIONS AND SMART LIBRARIES

摘要

HotSpot Wizard is an interactive web server for prediction of amino acid residues suitable for mutagenesis and construction of libraries of mutants with modified activity, specificity or stability [1]. Positions suitable for mutagenesis are evaluated based on protein structure using a combination of structural, functional and evolutionary information obtained from 7 internet databases and 22 computational tools [2].