SAMPLE PREPARATION FOR RAPID PATHOGEN DETECTION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING - (PPT)

摘要

BSIMAX - Bloodstream Infection testing on whole blood samples Collection - BD Vacutainer Glass Blood Collection Tubes with SPS (364960, Fisher catalog # 02-684-19) Sample preparation - In-system 1:3 dilution with tryptic soy broth (25% TSB-blood mixture), 1:1 RBC lysis buffer, pelleting and replenish with TSB Lysing - NaOH to break cell membrane and remove RNA binding proteins followed by HCL to adjust pH for RNA/DNA hybridization.