Evaluation of Nanofiber PLA Scaffolds Using Dry-and Wet-Electro Spinning Methods

摘要

Investigating the fabrication of a favorable scaffold is a continuing sconcern and an essential step within tissue engineering. An ideal scaffold should mimic the structure of extracellular matrix (ECM) which has considerable impacts on its function. To meet this demands, in this study, three-dimensional scaffolds by polylactic acid (PLA) polymer 10%, 13%, 15%, 17% and 20% (w/v) with a novel architecture produced by a wet-electrospinning process have been fabricated and compared with dry-electrospun scaffolds that have been developed for tissue engineering applications. In the wet-electrospinning method, an aqueous solution of sodium hydroxide (NaOH) (pH~13) has been used as the coagulation bath instead of the aluminum collector which is used in the dry method. Comparing with usual nanofiber non-woven PLA fabric prepared by a conventional electrospinning method, wet-electrospun spongiform PLA nanofiber scaffolds have low apparent density and high porosity. The porosity of the scaffolds has been examined in this two methods and their mechanical properties as well as their morphology are investigated. All in all, it is shown that the novel nanofiber fabrication system would be amazingly useful for the structure control of the nanofiber fabric.