Research on some factors affecting extraction of chlorophyll from mulberry leaves (Moras alba)

摘要

The purpose of this report is to investigate some factors affecting extraction of chlorophyll from mulberry leaves (Morus alba). In the three (3) types of solvents used (acetone, ethanol, and methanol), 90% acetone was the appropriate solvent to extract chlorophyll from mulberry leaves giving a total chlorophyll concentration of 0.341%, in which chlorophyll achieved a concentration of 63%. Some factors such as temperature, material/solvent ratio, extraction time were also investigated. This survey was studied for optimum use a Box-Wilson central composite design consisting of 03 independent variables (temperature, materials/solvent ratio and extraction time) and two dependent variables (total chlorophyll and chlorophyll a ratio. Second-order polynomial equations were obtained for the responses, which fitted well with the experimental data. Select the optimal conditions are T=26.75 °C, material/ solvent ratio 12.67 %, t = 24.3 h. Optimal results are found out conditions to obtain the highest chlorophyll total (Y_(1(opt))) = 0.354%) while chlorophyll a ratio at the desired concentration Y2> 60%.