Application of Humic Substances in Medicine: Basic Studies to Assess Pro-and Anticoagulant Properties of Humic Acids

摘要

This study was performed in order to evaluate the dose-dependent effect of different humic acid (HA) preparations on blood coagulation. Using the thrombelastographic method, we examined HA from Altteich peat (eastern Saxony, Germany), sodium humate from an ombrotrophic mire in Mecklenburg-Western Pomerania, Germany, and the IHSS reference HA from Waskish peat (Minnesota, USA). The results revealed a strikingly bimodal behaviour of the tested HA: at high concentrations (250-1,000 μg/mL), they prolonged the reaction time, i.e. the period of time necessary until the beginning of clot formation, by 2-12-fold (anticoagulant effect); however, at low concentrations (0.9-31.3 ug/mL), they tended to reduce the reaction time by an average of 25% (procoagulant effect).