Cultivation and automated image analysis of hydrocarbon-tolerant marine cyanobacteria populations

摘要

In this paper we described the cultivation of phototrophic marine microorganisms from two different sources, with special aim to enrich the natural populations with respect to marine cyanobacteria that are able to tolerate (2-5 years) gasoline or diesel up to 5% (w/v). These enriched populations where studied with respect to morphology and physical relationship with hydrocarbon surfaces and with quantum dots using bright field and epifluorescence microscopy, coupled to automated digital image analysis.