Cost Effectiveness of Diagnostic Strategies Using Quantitative Realtime Polymerase Chain Reaction and Bacterial Culture to Identify Cows with Mycoplasma Mastitis During an Outbreak in a California Dai

摘要

Diagnostic strategies to detect contagious mastitis caused by Mycoplasma spp. in dairy herds during an outbreak have been minimally studied in terms of cost and sensitivity. The recent introduction of quantitative real-time PCR (qPCR; PathoProof Mastitis PGR Assay, Thermo Fisher Scientific) for Mycoplasma bovis allows for its use to rapidly detect infected cows using both individual cow samples or pooled milk samples. The objective of this cross-sectional study was to compare the cost effectiveness of strategies for identification of mycoplasma-infected cows in a California dairy herd of 1,210 cows during a mastitis outbreak.