METHOD FOR UNIVERSAL DETECTION AND QUANTIFICATION OF MYCOPLASMA (MOLLICUTES) 16S RDNA BY QUANTITATIVE POLYMERASE CHAIN REACTION AMPLIFYING A 1.5 KILOBASE FRAGMENT

摘要

The present invention relates to a method for detecting mycoplasma 16S rDNA 1.5 kilobase fragment amplified by quantitative PCR. One of the most critical limitation of the qPCR is the DNA fragment length to amplify. The existing qPCR methods does not permit the amplification of long DNA fragments. Here the inventors demonstrate for the first time the amplification of mycoplasma 16S rDNA 1.5 kilobase fragment using qPCR based on real- time polymerase chain reaction. In particular, the present invention relates to a method for universal detection and quantification of mycoplasma 16S rDNA 1.5 kilobase fragment in a sample comprising contacting said sample with degenerate primers amplified by quantitative PCR or qPCR and using a DNA loading probe.