Stability of Isolated Antibody-Antigen Complexes as a Predictive Tool for Selecting Toxin Neutralizing Antibodies

摘要

MOA of toxin-neutralizing anti-RTA antibodies may be stabilize the fold of the antigen. Unfolding is Irreversible - While we can not measure thermodynamic parameters we can still observe trends in the T_m-shifts of Ab-Ag complexes. Existing need for cell-free methods of determining toxin-neutralizing vs. non-neutralizing titers. MOA may apply to other proteins that undergo large conformational changes e.g. Viral envelop proteins. Antibody library screening.