Manufacture of PCL scaffolds through electrospinning technology to accommodate Triple Negative Breast Cancer cells culture

摘要

Two-dimensional (2D) cell culture structures are demonstrated to differ from the in vivo environment.Therefore,cells adopt a flattened morphology that may lead to a non-physiological behaviour.For instance,2D culture induces the differentiation of the cancer stem cells (CSCs),a tumorigenic cell niche whose study is crucial in tumors with a high relapse rate such as the triple negative breast cancer (TNBC).As an alternative,electrospun scaffolds which mimic the native extracellular matrix structure have emerged as a three-dimensional culture support.In this work,two different meshes of 7.5 and 15% of poly(s-caprolactone) (PCL) were fabricated,differentiating in their microstructure.Scaffolds exhibited similar DSC and TGA curves compared with raw PCL,indicating their purity.TNBC MDA-MB-468 cells were seeded on scaffolds and adopted a more elongated morphology when cultured on 15% PCL meshes.Hence,electrospinning PCL scaffolds may become a suitable tool to culture TNBC cells in a more physiological manner.