In Vitro Direct Plant Regeneration from Leaves of Blackberry Cultivar 'Boysenberry'

摘要

Plantlets were regenerated in vitro from leaves of blackberry hybrid cultivar 'Boysenberry'. Leaflets from 2-month-old in vitro-grown plantlets on MS medium with 0.1 mg/L TDZ were used as the expiants. After 20 days of culture, adventitious buds emerged at their petioles, performance of the 2~(nd) (from top) leaflet was best. The 2~(nd) leaflet from 1- to 5-month-old in vitro-grown plantlets were treated in the same way, and leaflets from 3-month-old plantlets showed best. The 2~(nd) leaflets of 3-month-old in vitro-grown plantlets were cultured on MS medium with 0.05 mg/L TDZ was the optimal medium for inducing adventitious buds without callus; MS medium with 1.5 mg/LKT + 0.1 mg/L NAA was the optimal medium for proliferation. The best rooting medium was 1/2 MS with 0.03 mg/L NAA.