IN VITRO DIRECT REGENERATION OF POLYPLOID CANNABIS PLANTS

摘要

The present invention relates to a new in vitro method for direct regeneration of a Cannabis sativa L. plant as well as the use of such method for micropropagation of selected elite clones belonging to Cannabis sativa L., development of polyploid Cannabis plants with enhanced levels of secondary metabolites, promote spontaneous rooting of in vitro regenerants in a shorter period of time than conventionally used methods, production of mutagenized plants and obtention of transgenic or gene-edited plants. The method advantageously comprises culturing selected explants such as apical meristems, true leaves, stem nodes and/or internodes from mature plants and cotyledons, hypocotyls and/or epicotyls from seedlings of short and neutral-day Cannabis plants in an appropriate culture medium.