Polyelectrolyte Multilayers As Antiadhesive Sacrificial Membrane Films: A Novel Approach to Virus Concentration for Detection

摘要

Prevention of disease outbreaks relies on the efficient detection of pathogens in drinking and recreational water. Because waterborne pathogens are typically present in low concentrations, a fast and reliable sample concentration process is a crucial step in the overall detection procedure. An emerging method of sample concentration for the detection of waterborne pathogens is crossflow membrane filtration, which offers several important advantages over traditional methods of pathogen concentration. First, membranes of an appropriate pore size can simultaneously concentrate a wide range of microbial pathogens from protozoa to bacteria to viruses without concentrating molecular toxins and low molecular weight qPCR inhibitorshttps://aiche.confex.com/aiche/nams 12/printabstractbook.cgi?password= - ENREF22. Second, crossflow decreases membrane fouling and results in higher rates of concentration. Third, in the presence of crossflow a larger fraction of viruses remains suspended in the membrane retentate throughout the concentration process facilitating virus recovery for subsequent detection. To prevent virus passage through and adsorption to the membrane and to further improve recovery, the membranes are usually "blocked" with a proteinaceous solution (e.g. calf serum) and sometimes are pretreated with a chemical dispersant. Despite these measures, however, the recovery of certain pathogens such as adenoviruses remains low and difficult to reproduce.