Immobilization of Pseudomonas sp. strain ADP: a stable inoculant for the bioremediation of atrazine

摘要

Storage and delivery of biological products are fundamental issues determining their effectiveness. For liquid cultures of Pseudomonas sp. strain ADP stored at 4 and 25°C, a 1 log reduction in cfu/mL occurs after approximately 4 and 2 weeks respectively. When immobilized onto natural zeolite and stored in open containers survival at 25°C is poor. However, when the cells are immobilized with xanthan gum and stored in closed containers, survival at 25°C is superior to cells stored at 4°C. The typeof growth medium, zeolite substrate and immobilization matrix excipients appear to play a role in the stabilisation of Pseudomonas sp. strain ADP. The bacterium remained viable and retained its ability to degrade atrazine for the complete test period of10 weeks at 25°C.