DIGITAL MICROFLUIDICS COUPLED TO NANOELECTROSPRAY IONIZATION MASS SPECTROMETRY FOR SUCCINYLACETONE ANALYSIS IN DRIED BLOOD SPOTS

摘要

We report a direct method for coupling digital microfluidics (DMF) with ESI-MS. The new digital microfluidic electrospray ionization (DMF-ESI) interface was formed by sandwiching a pulled glass capillary emitter between the two DMF substrates (a bottom substrate bearing an array of actuation electrodes and a top substrate bearing a counter electrode) with an inter-substrate gap selected such that the capillary emitter is immobilized without any external seals or gaskets. As proof of concept, on-chip processing and analysis of succinylacetone from dried blood spots was performed.