ON-CHIP SINGLE VESICLE ANALYSES OF ATP-BINDING CASSETTE (ABC) TRANSPORTERS

摘要

We developed a procedure to analyze the function of ATP-binding cassette (ABC) transporters quantitatively in a microfluidic device. Although we have succeeded in detecting ATP-dependent substrate transport by ABC-transporters in microfluidic channels [1], strong background fluorescence originated from vesicle-bound substrates was a problem for quantitative analyses of the interaction between ABC-transporters and drugs. We overcame this issue by using fluorescent substrates at very low concentration. Using this procedure, we present a first report of the on-chip accurate analyses of the interaction between several drugs and ABC-transporters, and single vesicle transport analyses representing the presence of functionally heterogeneous transporters in vesicles.