首页> 外文会议>International Conference on Miniaturized Systems for Chemistry and Life Sciences >A PLASMA LITHOGRAPHY MICROENGINEERED ASSAY FOR STUDYING ARCHITECTURE DEPENDENT WOUND HEALING OF ENDOTHELIAL CELLS
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A PLASMA LITHOGRAPHY MICROENGINEERED ASSAY FOR STUDYING ARCHITECTURE DEPENDENT WOUND HEALING OF ENDOTHELIAL CELLS

机译:用于研究内皮细胞建筑依赖性伤口愈合的等离子体光刻微能测定

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A plasma lithography microengineered wound healing assay is developed to study architecture and injury dependences of endothelial cell migration. We study the migration of endothelial cell monolayers with dimensions from single cell width to millimeters mimicking various cardiovascular structures. Furthermore, we investigate the role of mechanical injury using wound healing assays that introduce different degrees of cell injury. Remarkably, the endothelial migration rate is robustly maintained in various circumstances: line patterns with different widths and different levels of mechanical injury. Our results also reveal that the number of leader cells, which guide the healing process, is proportional to the width of the structure suggesting a novel multicellular mechanism for regulating the endothelial wound healing process.
机译:制定了一种等离子体光刻微能伤口愈合测定,以研究内皮细胞迁移的建筑和损伤依赖性。我们研究内皮细胞单层的迁移,从单个电池宽到尺寸到模拟各种心血管结构的毫米。此外,我们研究机械损伤使用引入不同程度的细胞损伤的伤口愈合测定的作用。值得注意的是,在各种情况下,内皮迁移率稳健地保持:线条图案具有不同的宽度和不同的机械损伤水平。我们的研究结果还表明,引导愈合过程的领导细胞数与结构的宽度成比例,这表明一种用于调节内皮伤口愈合过程的新型多细胞机制。

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