LENSFREE SUPER-RESOLUTION MICROSCOPY USING WETTING FILMS

摘要

Quantification and characterization of pathogenic parasites and bacteria in field-settings require high-throughput, highly-sensitive and portable microscopes. For this purpose, here we investigate the use of wetting films with lensfree super-resolution microscopy to significantly improve its imaging performance. Formation of a thin wetting film over the specimen (e.g., cells, bacteria) creates a micro-lens effect, providing up-to 4-fold improved signal-to-noise-ratio and contrast in our lensfree images, which enable the recovery of submicron (<0.5 μm) spatial features of the samples such as parasite flagella and Escherichia coli over a wide imaging-field-of-view of ~24 mm~2.