We investigate the use of wetting films to significantly improve the imaging performance of lensfree pixel super-resolution on-chip microscopy, achieving < 1 µm spatial resolution over a large imaging area of ~24 mm2. Formation of an ultra-thin wetting film over the specimen effectively creates a micro-lens effect over each object, which significantly improves the signal-to-noise-ratio and therefore the resolution of our lensfree images. We validate the performance of this approach through lensfree on-chip imaging of various objects having fine morphological features (with dimensions of e.g., ≤0.5 µm) such as Escherichia coli (E. coli), human sperm, Giardia lamblia trophozoites, polystyrene micro beads as well as red blood cells. These results are especially important for the development of highly sensitive field-portable microscopic analysis tools for resource limited settings.
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机译：我们研究了使用湿膜来显着改善无透镜像素超分辨率片上显微镜的成像性能，在约24 mm 2 的大成像区域上实现了<1 µm的空间分辨率。在样品上形成超薄的润湿膜可有效地在每个物体上产生微透镜效果，从而显着提高信噪比，从而改善无透镜图像的分辨率。我们通过无透镜片上成像对具有精细形态特征（例如，≤0.5µm尺寸）的各种物体（如大肠杆菌（E. coli），人类精子，贾第鞭毛虫蓝芽滋养体，聚苯乙烯微珠）进行无透镜片上成像验证了该方法的性能以及红细胞。这些结果对于开发用于资源受限设置的高灵敏度现场便携式显微分析工具尤为重要。
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