DESIGNING OF CONTINUOUS BIOHYDROGEN PRODUCTION BY ANAEROBIC CO-DIGESTION BASED ON PYROSEQUENCING ANALYSIS OF BACTERIAL COMMUNITY STRUCTURE

摘要

Previous studies of pyrosequencing analysis of microbial structure in co-digestion of crude cheese whey (CCW) with fruit-vegetables waste (FVW) allowed design a biohydrogen continuous system. To produce biohydrogen continuously, in hydrolytic stage was carried out in batch system using CCW and FVW as feedstock’s a carbon/nitrogen (C/N) ratio of 21. Slurry obtained from hydrolytic stage was fed slurry in a stirred-tank reactor (CSTR) in a hydrogenic stage. The performance of the hydrogen fermentation system was tested at different organic loading rates (OLR) from 21,900 to 155,800 g COD/L h by shortening the HRT (60 to 10 h) for 87 days. The optimum hydraulic retention time and organic loading rate were 17.5 h and 80.02 g COD/L d, respectively. Under these conditions, the highest volumetric production hydrogen rate (VPHR) and hydrogen yield were 11.02 mmol H2/L h, 800 mL H2/COD, respectively.