Sensitive heme sensor using fluorescent-labeled heme oxygenase-1

摘要

To detect free heme at low concentrations, we developed a sensitive heme sensor using fluorescent-labeled heme oxygenase-1, an enzyme which catalyzes the oxidative heme degradation and has a high affinity to heme. One of the sensors we prepared exhibited a good response against heme in a range of nanomolar level.