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Micropatterned Hepatocyte Culture Using Microstencil Technique

机译:微透明理由肝细胞培养使用微型药物技术

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摘要

A cell chip; in which the cells are micropatterned on a culture plate, is expected to apply various cell-based assays, such as drug screening, cell transfection, cell differentiation studies, and fundamental cell biological studies, because it can facilitate the observation of cell responses, including high-throughput screening. To develop such a cell chip, the formation of equal cell patterns on the culture plate is a key technology. Several cell patterning techniques for accelerating or inhibitingcell attachments have been reported, such as lithography, photo-reactive polymers, microcontact printing, and so on. However, these techniques involve the use of especial devices for microfabrication or surface modification and complicated processes in chip preparation. Therefore, a simple technique that can form the cell pattern on the culture plate will have advantages over previous methods.
机译:细胞芯片;其中细胞在培养板上进行微图案,预计将应用各种基于细胞的测定,例如药物筛选,细胞转染,细胞分化研究和基本细胞生物学研究,因为它可以促进细胞应答的观察,包括高吞吐量筛选。为了开发这种细胞芯片,培养板上的相同细胞图案的形成是关键技术。已经报道了用于加速或抑制链附件的几种细胞图案化技术,例如光刻,光反应性聚合物,微接触印刷等。然而,这些技术涉及使用特殊装置进行微生物或表面改性和芯片制备的复杂过程。因此,可以在培养基上形成细胞图案的简单技术将具有优于先前的方法。

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