Swellscreen - Rapid Baculovirus Titration Method in Microplate Format

摘要

A fast and convenient method for baculovirus quantification in microplate format has been developed. The cell diameter was chosen as the measuring parameter, since insect cells are enlarged upon infection, and swelling is proportional to the amount of virus particles in the solution (Liu, 2004). Insect cells were seeded into a 96-well microplate, infected with serial dilutions of bac-uloviruses, and incubated at 27癈. After 24 h, the cell diameter average in each well was determined in an automated image-based screening system. Dose response curves resulting from infections of viruses with known and unknown titles, respectively, were compared, and the titres of the unknown viruses calculated from the midpoint shift. This approach enabled simultaneous quantification of five viruses in one 96-well plate and could be carried out in the presence of cell growth media.