An Avian Cell Line for Production of Highly Attenuated Viral Vectors

摘要

AGE1 CR is a designed permanent cell line from duck embryos for pharmaceutical vaccine and viral vector generation. This line is adapted to proliferation in medium free of animal-derived components to provide a convenient system for industrial vaccine production. In this system, a burst size of up to 5000 is obtained for MVA within 48 h post infection. To further improve yield of viral vectors an additional transgene was stably introduced into the cell line, giving AGE1 CR.pIX. Surprisingly, although yield of MVA is further increased (depending on production parameters 3-fold to 20-fold, with a currently maximum burst size exceeding 16000), the sensitivity of AGE1 CR.pIX to inducers of the antiviral response is elevated, the infectious cycle is not accelerated and cellular proliferation rate is decreased.