Extraction of THC and metabolites from Urine and Plasma using Supported Liquid Extraction (SLE) prior to LC-MS/MS Analysis

摘要

Recoveries of >80percent were demonstrated from both plasma and urine. Hexane elution yielded high recoveries of the less polar analytes whilst more polar metabolites demonstrated low extraction efficiencies. EtOAc, DCM and MTBE demonstrated high extraction efficiencies for both matrices, however, matrix pH was an important factor. Higher extraction efficiencies may be obtained using mixed elution solvents or subsequent elutions of hexane followed by ethyl acetate, DCM or MTBE to incorporate both the non-polar and more polar functionalities.