MS-based Approach for System Analysis of Protein Turnover

摘要

Our data on 30 different mutants validate the concept and reveal the power of the approach, quantitating nearly 100,000 high confidence turnover comparisons. This T-map reflects the system-wide degradation network in eukaryotic cells. The results from this research will allow us generating new hypotheses on clients for specific turnover pathways and reveal classes of protein cargoes using a pathway. This will lead to new hypotheses for homeostatic control of proteins and reveal which factors work together in turnover pathways.