GC-MS-based metabolomics analysis of 3,2-trans-enoyl-CoA isomerase knockdown mutants in human hepatoma cells

摘要

732 features were identified by matching the GC-MS data to entries in the Fiehn Metabolomics RTL Library. 37 metabolites were identified as having significant differences (q < 0.05) in abundance among the four cell types based on ANOVA, while a G-test analysis identified an additional 64 metabolites as significantly different (p < 0.05) based on occurrence. Most amino acids and their derivatives, fatty acids, sugars, and vitamins were increased in DCI-1 knockdown cells relative to the other three cell types, while nucleobases and aromatic metabolites were decreased. Increases in amino acids and intermediates of urea synthesis are consistent with multiple fatty acid oxidation deficient mouse models and suggest an increased dependence on amino acids for energy production in the absence of mitochondrial beta-oxidation of fatty acids.