Trivalent metal cationization of acidic fibrinopeptide b produces multiply charged ions of 2+, 3+, and 4+ by ESI. Greater than 90% dissociation of [M+Met+H]~(4+) for most lanthanide metals Highest ETD efficiency for [M+Met+H]~(4+). Readily interpretable ETD spectra. ETD of [M+Pr+H]~(4+) produced cleavage at every backbone residue and yielded product ions which included primarily metallated and non-metallated c_n and z_n-type ions. Removal of the cyclized N-terminus (pGlu) in the peptide did not affect the formation of [M+Met-H]~(2+), [M+Met]~(3+), or [M+Met+H]~(4+). Removal of the C-terminal arginine (Arg) in the peptide changed the distribution of the intensities of the multiply charged precursors, but high efficiency ETD spectra were still obtainable.
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机译：酸性纤维素肽B的三价金属阳离子化产生2+，3+和4+的多元带电离子。大于90％的[M + Met + H]〜（4+）的离解，用于大多数镧系金属最高的ETD效率，用于[M + MET + H]〜（4+）。易于解释的ETD光谱。 [M + Pr + H]〜（4+）的ETD在每个骨干残留物处产生裂解，并得到产物离子，其主要是金属化的和非金属的C_N和Z_N型离子。在肽中除去环状的N-末端（PGLU）不影响[M + MET-H]〜（2+），[M + MAT]〜（3+）的形成，或[M + MET + H. ]〜（4+）。除去肽中的C末端精氨酸（Arg）改变了倍增带状前体的强度的分布，但仍然可以获得高效ETD光谱。
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