Development and Application of a Comprehensive Isotope-labeled Peptide Library to Quantify the Twelve Signal Transduction Pathways of Carcinogenesis

摘要

Development of a SIS library for twelve pathways involved in cancer using 100 proteins Quantitation of 93 proteins across twelve key pathways. Significant changes were seen in the TNF-α/NFκB induced apoptosis pathway (Figure 3 and Table 2) as has been noted previously. (1) Taxol induced expression of TNF-α, promoting TNFR2 receptor that increasing activation of NFκB and AKT1 while inhibiting phosphorylation of BAD, promoting cell proliferation and suppressing apoptosis. Significant decreases were detected for ATR and ATM (Figure 3 and Table 2), both key players in double strand break DNA repair and both the G_1 and G_2 checkpoints. (1) Inhibition of both ATM and ATR has been used to re-sensitized cisplatin resistant cells (2) Further studies are ongoing to investigate the role of these proteins in both taxol-and cisplatin-resistance.