A porous AgNP-impregnated thin film prepared by a sol-gel method is demonstrated as a flexible platform for LDI-MS of analytes of various chemical classes. The AgNP-impregnated thin film absorbs incoming photons, resulting in desorption and ionization of analytes, which are observed as Ag+ adducts. This investigation focuses on the analysis of several non-olefin analytes, specifically, peptides and several phospholipids/triglycerides with fully saturated hydrocarbon chains. Moreover, the platform displayed an excellent shelf life and retained its optical properties for over a year without compromising LDI-MS function, an appealing quality from a diagnostic standpoint where minimal sample preparation is often required for rapid analysis. Methods for the incorporation of iron oxide thin films for phosphorylated peptide enrichment are briefly discussed. The preliminary study demonstrates that phosphorylated peptides are preferentially bound to the surface of the thin film, however, optimization of the protocol is currently being investigated.
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