Toxicological Analysis of Whole Blood Samples Using Automated SPE/HPLC/MS/MS

摘要

In this comparison of ITSP with traditional sample preparation and confirmation techniques, ITSP has proven itself to be sufficiently robust to enable analysis of post mortem blood specimens even after extended storage of specimens. Appropriate sample dilution prior to extraction and the use of positive pressure for sample application and elution resulted in an automated method capable of extracting difficult biological specimens. Use of automated sample preparation coupled with quantitation/confirmation by LC/MS/MS should reduce analyst work load related to sample preparation thereby reducing turn around time and allowing the analyst to concentrate on other tasks. Future work should involve a larger set of actual cases and provide evaluation of additional drugs and drug classes as well as the ability of the ITSP system to process additional specimen types.