Characterization of flagellar hook protein from spirochetes using tandem mass spectrometry

摘要

Tandem mass spectrometry conclusively identifies the HMWC of spirochete T. denticola as composed exclusively of FlgE. Tandem mass spectrometry suggests peptide T15 is important to the cross-linked HMWC species. Tandem mass spectrometry identifies a likely cross-linked peptide species from T13/14 and T15 at m/z 3731.9397 u. Mutation of selected amino acids on T15 suggests the roles of Asp~(181), Asn~(179) and Cys~(178) in the biochemical mechanism of isopeptide bond formation and covalent cross-linking of FlgE in the HMWC.