Large Scale Analysis of Protein-Ligand Binding Interactions using a SILAC-Based Proteomics Platform

摘要

Thermodynamic analyses can provide important information about protein folding and ligand binding interactions A Mass spectrometry based protocol, termed SPROX, has been developed for making thermodynamic measurements on proteins and protein-ligand complexes. Described here is a proteome-wide ligand binding assay that combines SPROX with Stable Isotope Labeling by Amino acids in Cell Culture (SILAC-SPROX) to expand the scope of SPROX.