Serial enrichment of post-translationally modified peptides enables deep and quantitative analysis of the proteome, phosphoproteome, ubiquitinome, and acetylome

摘要

We have developed a reproducible workflow that allows for deep proteome and multiple PTM analyses from the same sample (1) Basic reversed-phase chromatography facilitates even and unique fractions of modified and unmodified peptides (2) Serial enrichments do not affect downstream enrichments Deep proteome coverage reveals a small subset of proteins upregulated due to treatment with proteasome inhibitor Velcade Velcade treatment profoundly affects protein ubiquitination and upregulates phosphorylation of cell cycle and transcription regulator proteins