A Complete Workflow Solution for Monoclonal Antibody Glycoform Characterization Combining a Novel Glycan Column Technology and Bench-Top Orbitrap LC-MS/MS

摘要

GlycanPac AXH-1 column separates glycans with unique selectivity based on charge, size and polarity. The GlycanPac AXH-1 columns are compatible with MS instruments. LC-ESI-FTMS or FT-MS/MS analysis of both native and labeled glycans from proteins and antibodies were carried out successfully using GlycanPac AXH-1 columns. Confident identification and structural confirmation of glycans can be achieved using high-resolution HCD MS/MS which produces an informative spectrum containing glycosidic and cross ring fragment ions. A complete workflow solution was developed for glycan profiling combining the unique GlycanPac AXH-1 column technology and a bench-top Orbitrap LC-MS/MS. This workflow was applied to characterize a monoclonal antibody glycoforms. Confident identification and structural confirmation was achieved for released glycans from the monoclonal antibody.