Towards a Comprehensive Understanding of Platelet Activation and Platelet-monocyte Interaction: Multiple Proteomic Approaches in the Study of Atherosclerosis

摘要

Multiple proteomic methods have been developed and applied on a model system studying platelet-monocyte interaction in the context of atherosclerosis. Monocyteactivated by platelet releasate leads to a systematic proteomic change over a long span of time. This includes short -term phosp horylation of signaling proteins, e.g. Rho GTPases, increased Reactive Oxygen Species production mediated by NADPH Oxidase and long-term up -regulation of membrane and glycoproteins with various biolo gical functions. This systematic proteomic approach will be applied on more complex systems in the future, e.g. platelet -monocyte aggregate, to achieve better understanding of the mechanism behind atherosclerosis leading to the discovery of potential drug target.