Overcoming Challenges of Protein Sample Preparation for Food Allergen Analysis

摘要

The Perfinity iDP automates and integrates protein digestion, desalting and LC separation while adding speed, quality and value to MS-based assay development. Complete, rapid digestion of the Ara h1 protein was demonstrated reproducibly and common tryptic peptides of Ara h1 protein were successfully identified on the Perfinity iDP. The Perfinity iDP coupled to LCMS-IT-TOF was able to generate linear calibration curves for spiked food samples indicating quantitative recovery of trypsic peptides. These factors suggest that this new platform is ideal for food safety applications.