Catching Histone Modifying Enzymes in Action

摘要

"M4K" measures concerted methyltransferase and demethylases activities. Bi-directional antagonism between K27 and K36 methylation in vivo proven general. This novel quantitative technique provides new approach to investigate histone methylation kinetics and cross-talk network among marks. Insight for a specific methyltransferase (MMSET) and demethylase (UTX) using cell lines carrying overexpression or re-current mutations (possible cancer driver mutation).