Analysis of Intact Proteins in Complex Mixtures Using an LC-LTQ-Orbitrap

摘要

HPLC columns are not yet as effective for proteins as for peptides Averaging scans is beneficial for extending the mass range but significantly increases the duty cycle. Fractionation of proteins via 2-dimensional LC or molecular weight cutoff filter resulted in a higher number of identifications In our hands CID activates proteins more effectively and more reliably than HCD or ETD. In application of MC-7 cytosol, twenty two proteins were found to be modified.