1. Three Three crosslinked crosslinked lysine pairs were identified in both lysine pairs were identified in both monomericmonomeric and dimeric dimeric PSS2 molecules, while the PSS2 molecules, while the crosslinking crosslinking between K252 and K351 was identified only in between K252 and K351 was identified only in the dimeric dimeric PSS2 structure. PSS2 structure. 2. The spatial distance between the The spatial distance between the crosslinked crosslinked lysine pairs is lysine pairs is within 24 A. 3. K20, K133 and K249 are easily accessible by the K20, K133 and K249 are easily accessible by the crosslinkercrosslinker.. 4. K252 K252--K351 linkage K351 linkage shown only in shown only in dimer dimer may indicate the may indicate the involvement of involvement of dimer dimer formation for PSS2 function. formation for PSS2 function. 5. The data provide the first experimental evidence regarding The data provide the first experimental evidence regarding the 3D structure of the integral membrane protein PSS2. 6. The approach should be useful The approach should be useful to probe the molecular to probe the molecular basis basis for the for the substrate specificity and regulatory function of substrate specificity and regulatory function of PSS2 PSS2 in controlling membrane PS, an important modulator for controlling membrane PS, an important modulator for several signaling pathways.
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