Characterization of Oxidative DNA-Protein Crosslinking at Guanine by Mass Spectrometry

摘要

Reactive oxygen can modify DNA leading to permanent mutations and forming cytotoxic replication stops. Increasing the amount of reactive oxygen has been linked to aggressive forms of cance r along with reductions in tumor suppression. Our research shows that cross-linking readily forms in biologically relevant oxidation systems. Yet despite its obvious importance, oxidative DNA-protein cross-links have received little attention when compared to other types of DNA damage and remains one of the least understood types of oxidative lesion. Possible cross-links to lysine are shown below.