A quantitative MS-based proteomic and phosphoproteomic analysis of VSV-infected K562 cells

摘要

A robust, cost -effective quantitative shotgun proteomic and phosphoproteomic method was developed and used to analyze the effects of infection by the oncolytic VSV on a leukemic (K 562) cell line. Of 699 proteins and 100 phosphorylation sites identified, 53 proteins and 8 phosphorylation sites were found to be upregulated and 11 proteins and 9 phosphorylation sites were found to be downregulated. SAX was found to be more efficient than SCX at fractionating peptides, although the loss of basic residues after dimethyl labeling was found to decrease separation efficiency compared to their unlabeled counterparts.