Multiplexed Analysis of Cysteine-Containing Peptides in Biological Samples Using Cysteine-Specific Tandem Mass Tags

摘要

The application of isobaric tags for peptide quantification in complex biological samples allows multiplexing of analyses and increases the throughput. Using this approach quantitative information is obtained from acquired MS2 data [1]. However, co-isolation of nearly isobaric background ions tremendously impairs the quantification performance. The reduction of sample complexity by fractionation techniques greatly prevents such interferences and is required to detect low-abundance proteins. In addition to the commonly used amine-reactive tandem mass tags (TMT) reagent its thiol-specific TMT analog facilitates selective enrichment of cysteinyl peptides via anti-TMT immunoaffinity chromatography rendering complexity reduction and improved quantification [2].